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Image Search Results
Journal: Bio-protocol
Article Title: FRET Reporter Assays for cAMP and Calcium in a 96-well Format Using Genetically Encoded Biosensors Expressed in Living Cells
doi: 10.21769/bioprotoc.3641
Figure Lengend Snippet: A. Raw data obtained from a single well on the reading plate illustrates the time-dependent reciprocal increase and decrease of 485 and 535 nm H188 relative fluorescence unit (RFU) emission intensity in response to application of 300 pM glucagon to HEK293-hGCG-R-H188 c10 cell suspensions. Administration of the test substance is indicated by the vertical arrow, here and in subsequent figures. B. Determination of the single well percent change of H188 485/535 nm emission ratio (% ΔFRET) calculated using the single well raw data illustrated in panel 2A. An increase of the 485/535 nm FRET ratio signifies an increase of [cAMP]. A value of 0 is obtained after baseline subtraction of the FRET ratio at time point 0. A value of 70 corresponds to a 70% increase of FRET ratio relative to baseline. C. Glucagon produced a time-dependent and dose-dependent increase of the 485/535 nm H188 FRET ratio. Data for each time point is the mean % ΔFRET ± S.D. for n = 12 wells. D. Non-linear regression analysis of the data illustrated in panel 2C demonstrated that the EC50 for glucagon action in this cell line was 8.1 pM. Non-linear regression analysis was performed with Origin 8 software using the highest ΔFRET values for each data point. Non-linear reduction can also be performed with GraphPad Prism 8. For instruction in how to obtain values for time-dependent, dose-dependent and non-linear reduction with SoftMax Pro 7.1 see Video 2.
Article Snippet: list-behavior=enumerated prefix-word= mark-type=upper-alpha max-label-size=0 Instrument preparation: see Video 1 FlexStation 3 and
Techniques: Fluorescence, Produced, Software
Journal: Bio-protocol
Article Title: FRET Reporter Assays for cAMP and Calcium in a 96-well Format Using Genetically Encoded Biosensors Expressed in Living Cells
doi: 10.21769/bioprotoc.3641
Figure Lengend Snippet: A. Raw data obtained from a single well on the reading plate illustrates the time-dependent reciprocal decrease and increase of 485 and 535 nm YC3.60 relative fluorescence unit (RFU) emission intensity in response to application of CCK2 receptor agonist DG2 (300 nM) to suspensions of HEK293-hCCK2R c29 cells adenovirally transduced with YC3.60. B. Determination of the single well percent change of YC3.60 535/485 nm emission ratio (% ΔFRET) calculated using the single well raw data illustrated in panel 3A. An increase of the 535/485 nm FRET ratio signifies an increase of [Ca2+]i. C. DG2 produced a time-dependent and dose-dependent increase of the 535/485 nm YC3.60 FRET ratio. Data for each time point is the mean % ΔFRET ± S.D. for n = 6 wells. D. Non-linear regression analysis of the data illustrated in panel 3C demonstrated that the EC50 for DG2 action in this cell line was 604 pM. Non-linear regression analysis was performed with Origin 8 software using the highest ΔFRET values for each data point. Non-linear reduction can also be performed with GraphPad Prism 8. For instruction in how to obtain values for time-dependent, dose-dependent and non-linear reduction with SoftMax Pro 7.1 see Video 2.
Article Snippet: list-behavior=enumerated prefix-word= mark-type=upper-alpha max-label-size=0 Instrument preparation: see Video 1 FlexStation 3 and
Techniques: Fluorescence, Transduction, Produced, Software